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KMID : 0903619980390030371
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Journal of the Korean Society for Horticultural Science
1998 Volume.39 No. 3 p.371 ~ p.375
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Separation of Cytokinins in Ginseng Seeds during After - ripening by HPLC
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Abstract
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The contents of endogenous cytokinins in after-ripening ginseng (Panax ginseng C.A. Meyer) seeds were quantified with radish cotyledon expansion bioassay and HPLC with reversed C-18 column. The cytokinin contents increased significantly during the stratification and reached maximum by the end of the stratification period. Contents then rapidly decreased during the subsequent chilling period and remained very low at the stage of radicle emergence. Dihydrozeatin (DHZ) was the major cytokinin in ginseng seeds consisting 36-60% of the total cytokinins. Dihydrozeatin riboside (DHZR) and trans-zeatin (traps-Z) were found in relatively large quantity. The contents of traps-zeatin riboside (traps-ZR) showed rather steady increase during the seed stratification and chilling period, and became the most abundant cytokinins in germinating ginseng seeds. cis-Zeatin (cis-Z) and cis-zeatin riboside (cis-ZR) were also detected during the period from seed stratification to chilling. The contents of isopentenyladenine (iP) and isopentenyl adenosine (iPA) were very low and often found in trace amounts only. The results suggest that cytokinins are closely related with the germination of ginseng seed and DHZR, and possibly t-ZR is the most important cytokinins involved in the germination process.
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